Biological safety cabinet steps
2021-02-01
1. All the items required for this operation should be moved into the safety cabinet before operation to avoid frequent breakage of the airflow through the air curtain; and disinfect the surface with 70% alcohol before removing it to remove the pollution. 2. Turn on the fan for 5 to 10 minutes, and then perform the experimental operation after the air in the cabinet is purified and the airflow is stable. Slowly extend your arms into the safety cabinet for at least 1 minute to stabilize the airflow in the cabinet before operating. 3. Items not related to this experiment are not placed in the safety cabinet. The items in the cabinet should be placed in a clean area, semi-contaminated area and contaminated area. The items are easy to use during operation and there is no intersection between the three areas. Items should be placed as far back as possible, but the airway opening should not be blocked to avoid interference with normal airflow. 4. Operation should be carried out from the cleaning area to the contaminated area to avoid cross-contamination. To prevent possible spills, a towel or gauze soaked with disinfectant can be placed on the countertop, but the safety cabinet grille should not be covered. 5. During the operation of the cabinet, it is strictly forbidden to use an open flame such as an alcohol lamp to avoid the generated heat generating airflow, which may interfere with the airflow in the cabinet; and the open flame may damage the HEPA filter. 6. Minimize the movement of people behind the work and quickly switch the door to prevent the airflow in the safety cabinet from being unstable. 7. During the experimental operation, the glass window should not be opened, and the operator's face should be above the working window. When operating in the cabinet, the action should be gentle and soothing to prevent airflow in the cabinet. 8. The safety cabinet should be tested and maintained regularly to ensure its normal operation. Once the safety cabinet is found to be working abnormally, stop working immediately, take appropriate measures, and notify relevant personnel. 9. After the work is completed, close the glass window, keep the fan running for 10 to 15 minutes, and turn on the UV lamp for 30 minutes. 10. The safety cabinet should be cleaned and disinfected regularly. The contaminants in the cabinet can be wiped with 2% 84 disinfectant after the work is completed and the UV lamp is disinfected. The outer surface of the cabinet should be wiped with 1% 84 disinfectant per day. 11. The items used in the cabinet should be taken out after disinfection to prevent the pathogenic microorganisms from being taken out and polluting the environment.
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